![]() Guda and me help each group with password. Subsequently, ribosomal RNA (rRNA) sequence was predicted using RNAmmer version 1.2 and the predicted rRNA sequence was compared with NCBI BLAST database as well as EzTaxon database to verify the bacterial identity (6-8). Logging on Open up a Remote Desktop, pairs IP, password, id will be handed out in class Right-click CLC GWB and run as Administrator Let Dr. Thus, NGS technology has proven to be competent and a rapid in-silico alternative to resolve the complete mitochondrial genome sequence, thereby reducing labors and time.ĬLC-Bio Labeo rohita Roche 454 GS FLX mitogenome next-generation sequencing. using CLC Genomics Workbench version 7.0.4 (4, 5). This study was done to determine the feasibility, accuracy and reliability of low-depth sequence data obtained from NGS platform as compared to the Sanger sequencing. The mitogenome in the present investigation has 99% similarity with that of previously reported mitogenomes of rohu and this is also evident from the phylogenetic study using maximum-likelihood (ML) tree method. The gene order and organization are similar to most vertebrates. It comprised of 13 protein-coding genes, 22 tRNAs, 2 rRNAs and 1 putative control region. New workflow elements together with enhanced metadata capabilities allow. Features for the Genomics Workbench are described below: Analyzing and visualizing Next Generation Sequencing data, incorporates cutting-edge technology and algorithms, while also supporting and integrating with the rest of your typical NGS workflow. Workflows can combine quality control steps, adapter trimming, read mapping, variant detection, and multiple filtering and annotation steps into a pipeline you can share with colleagues and execute with just one click. The CLC Genomics Workbench is the client software for the CLC Genomics Server. rohita to be 16 606 bp long (accession No. QIAGEN CLC Genomics Workbench is developed to support a wide range of NGS bioinformatics applications. Low-depth shotgun sequencing using Roche 454 GS FLX (Branford, Connecticut, USA) followed by de novo assembly in CLC Genomics Workbench version 7.0.4 (Aarhus, Denmark) revealed the complete mitogenome of L. In the present study, we used in-silico approach to resolve complete mitochondrial genome of rohu. Labeo rohita, popularly known as rohu, is a widely cultured species in whole Indian subcontinent.
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